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Comparison of gene expression of WT and PerM mutant M. tuberculosis H37Rv grown in high and reduced magnesium

ABSTRACT: M. tuberculosis membrane protein PerM is required for persistence in chronic mouse infection. In vitro, the perM mutant requires increased magnesium for replication and survival, with elongation and lysis in low-magnesium media. To gain insight into the function of PerM, we compared the transcriptomes of wt and perM mutant Mtb grown in high (2000 μM) and reduced (250 μM) Mg2+ in three independent experiments. Transcriptome analysis of the perM mutant grown in reduced magnesium revealed upregulation of cell division and cell wall biosynthesis genes. Bacteria were grown for 5 days in Sauton’s media containing 250 or 2000 μM MgCl2 and 0.05% Tween 80. Three independent experiments were performed.

ORGANISM(S): Mycobacterium tuberculosis  

SUBMITTER: Sabine Ehrt  Nichole Goodsmith    

PROVIDER: E-GEOD-57263 | ArrayExpress | 2015-01-10



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