Transcriptomics,Multiomics

Dataset Information

91

Gene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [RNA-seq_differentiation]


ABSTRACT: Polycomb group (PcG) proteins are required for normal differentiation and development, and their activity is found deregulated in cancer. PcG proteins are involved in gene silencing, however, whether they initiate or maintain transcriptional repression is a subject of debate. Here, we show that knockout of the Polycomb repressive complex 2 (PRC2) does not lead to significant gene expression changes in mouse embryonic stem cells (mESCs), and that it is dispensable for initiating silencing of target genes during differentiation. Transcriptional inhibition in mESCs is sufficient to induce genome-wide ectopic PRC2 recruitment to endogenous PcG target genes found in other tissues. PRC2 binding analysis shows that it is restricted to nucleosome-free CpG islands (CGIs) of un-transcribed genes. Our results show that it is the transcriptional state that governs PRC2 binding, and we propose that it binds by default to non-transcribed CGI genes to maintain their silenced state and to protect cell identity. RNA-seq time-course experiments during in vitro differentiation (0h, 24h, 48h and 72h) of Mus musculus wild-type E14 and Suz12 knock-out Embryonic Stem Cells with two biological replicates per condition.

ORGANISM(S): Mus musculus  

SUBMITTER: Kristian Helin  Eva M Riising   Benjamin Leblanc   Itys Comet    

PROVIDER: E-GEOD-58017 | ArrayExpress| 2014-09-06

SECONDARY ACCESSION(S): GSE58017SRP042333PRJNA248720

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Gene silencing triggers polycomb repressive complex 2 recruitment to CpG islands genome wide.

Riising Eva Madi EM   Comet Itys I   Leblanc Benjamin B   Wu Xudong X   Johansen Jens Vilstrup JV   Helin Kristian K  

Molecular cell 20140703 3


Polycomb group (PcG) proteins are required for normal differentiation and development and are frequently deregulated in cancer. PcG proteins are involved in gene silencing; however, their role in initiation and maintenance of transcriptional repression is not well defined. Here, we show that knockout of the Polycomb repressive complex 2 (PRC2) does not lead to significant gene expression changes in mouse embryonic stem cells (mESCs) and that it is dispensable for initiating silencing of target g  ...[more]

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