Transcriptomics

Dataset Information

6

A Novel Human Gastric Primary Cell Culture System for Modeling Helicobacter Pylori Infection In Vitro


ABSTRACT: We identified specific long-term culture conditions that allow the formation of 3-dimensional human gastric spheroids, which can be differentiated by withdrawal of Wnt3A and R-spondin1 to human gastric organoids. These cultures can expand indefinitely and exhibit important characteristics of human stomach tissue. Furthermore, these 3-dimensional cultures can be transferred into 2-dimensional primary epithelial cell layers, which can be successfully infected with H. pylori and thereby provide ideal conditions to study infections in vitro. Microarray experiments were performed as dual-color hybridizations on Agilent human whole genome catalog 44K arrays. To compensate for dye-specific effects, a dye-reversal color-swap was applied.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hans-Joachim Mollenkopf   Philipp Schlaermann  Thomas Meyer 

PROVIDER: E-GEOD-58473 | ArrayExpress | 2014-12-10

SECONDARY ACCESSION(S): GSE58473PRJNA252707

REPOSITORIES: GEO, ArrayExpress

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