Genomics

Dataset Information

297

Transcriptional and epigenetic program changes of induced beta cells over time [RRBS-seq]


ABSTRACT: Direct lineage conversion is a promising approach to generate therapeutically important cell types for disease modeling and tissue repair. However, it is often unclear whether lineage-reprogrammed cells remain stable long-term and whether the properties of the reprogrammed cells evolve over time. Here, using an improved method of converting pancreatic acinar cells to beta-cells, we show that induced beta-cells persist in the adult pancreas for up to 14 months and form islet-like structures. Detailed analyses of induced cells over 7 months reveal that global DNA methylation changes occur rapidly whereas transcription network remodeling evolves over two months to resemble that of endogenous beta-cells and then stabilizes thereafter. Progressive gain of beta-cell function by converted cells during the 7 month period coincides with both transcriptional changes and the formation of islet-like structures. These studies demonstrate the ability of lineage-reprogrammed cells to achieve a stable state and identify key cellular and molecular milestones during their long-term evolution. Acinar cells and beta cells were collected as control, as well as induced beta cell samples at day 10, day 30, day 60, and 7 months

ORGANISM(S): Mus musculus  

SUBMITTER: Yingying Zhang   Jiang Zhu  Weida Li  Qiao Zhou  Kendell Clement 

PROVIDER: E-GEOD-59615 | ArrayExpress | 2014-11-15

SECONDARY ACCESSION(S): GSE59615SRP044672PRJNA255762

REPOSITORIES: GEO, ArrayExpress, ENA

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