Transcriptomics

Dataset Information

302

CapStarr-seq: a high-throughput method for quantitative assessment of enhancer activity in mammals


ABSTRACT: Here we developed CapStarr-Seq, a novel high-throughput strategy to quantitatively assess enhancer activity in mammals. This approach couples capture of regions of interest to previously developed Starr-seq technique. Extensive assessment of CapStarr-seq demonstrated accurate quantification of enhancer activity. Furthermore, we found that enhancer strength correlates with binding complexity of tissue-specific transcription factors and super-enhancers, while additive enhancer activity isolates key genes involved in cell identity and function. CapStarr-seq analysis in P5424 cell line (2 replicates), 3T3 cell line and in the plasmid library before (Input) and after transfection

ORGANISM(S): Mus musculus  

SUBMITTER: Joaquin Zacarias   Laurent Vanhille  Ahmad Maqbool  Salvatore Spicuglia  Jean-Christophe Andrau  Nicolas Fernandez  Aurélien Griffon  Benoit Ballester 

PROVIDER: E-GEOD-60029 | ArrayExpress | 2015-04-09

SECONDARY ACCESSION(S): SRP045188GSE60029PRJNA257302

REPOSITORIES: GEO, ArrayExpress, ENA

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