Transcriptomics

Dataset Information

4

Transcription profiling of mouse liver exposed to 24h cytokine starvation vs. treatment with TNF alpha


ABSTRACT: Pathogenesis of atherosclerosis results from the interactions between disrupted lipid homeostasis and immune response but the molecular bridges between the major players are still a matter of controversy. We investigated the systemic effect of inflammatory cytokine tumor necrosis factor alpha (TNF-α), a well established anorexia agent, in livers; of mice exposed to 24h-cytokine/starvation. 10% of genes of the Agilent 10kb cDNA array is detected above level of detection in the mouse liver. 12% of these is modulated upon starvation while 22% is altered by TNF-α. Experiment Overall Design: Twelve mice (C57BL/6, Harlan) were randomly assigned into three groups. Three animals were i.v. injected by human recombinant TNF-α (30 µg/animal), a dose that is known to have a significant effect in Experiment Overall Design: mice without leading to mortality. Food was withdrawn after application of TNF-alpha (TNF/starvation group). Further, for three animals which were treated with corresponding volume (200µl) of saline a food was withdrawn (starvation group). Three animals from control group had free access to food throughout the study. Mice were sacrificed 20 hours later. Livers for RNA analysis were stored in RNAlater (Quiagen) according to manufacture's instruction. RNA of individual treated mouse was hybridized versus control sample which is obtained by pooling of control RNAs.

ORGANISM(S): Mus musculus  

SUBMITTER: Drago Kuzman  

PROVIDER: E-GEOD-6317 | ArrayExpress | 2008-06-14

SECONDARY ACCESSION(S): GSE6317PRJNA100529

REPOSITORIES: GEO, ArrayExpress

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Publications

The Sterolgene v0 cDNA microarray: a systemic approach to studies of cholesterol homeostasis and drug metabolism.

Rezen Tadeja T   Juvan Peter P   Fon Tacer Klementina K   Kuzman Drago D   Roth Adrian A   Pompon Denis D   Aggerbeck Lawrence P LP   Meyer Urs A UA   Rozman Damjana D  

BMC genomics 20080211


BACKGROUND: Cholesterol homeostasis and xenobiotic metabolism are complex biological processes, which are difficult to study with traditional methods. Deciphering complex regulation and response of these two processes to different factors is crucial also for understanding of disease development. Systems biology tools as are microarrays can importantly contribute to this knowledge and can also discover novel interactions between the two processes. RESULTS: We have developed a low density Sterolge  ...[more]

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