Transcriptomics

Dataset Information

298

MicroRNAs and isomiRs repertoire in poricne endometrium revealed by Illumina sequencing


ABSTRACT: Recently, microRNAs (miRNAs), known to modulate gene expression through post-transcriptional mechanisms, were implied in regulation of early pregnancy events including maternal recognition of pregnancy and implantation. To characterize complex transcriptomic changes in expression of miRNAs in pregnant and cyclic endometria collected on days 12, 16 and 20 was analyzed using Illumina deep sequencing. This research showed repertoire of pregnancy-related miRNAs in porcine endometrium during initial stages of conceptus implantation and during the estrous cycle and sheds light on miRNA-mediated gene expression regulation mechanism at the maternal-conceptus interface. miRNAs and isomiRs expression profiles were analyzed in samples collected from porcine endometrium on Days 12, 16 and 20 of pregnancy and estrous cycle. Each group was represented by 4 to 6 samples. Generated libraries were run in multiplex using specific barcodes to differentiate samples. Illumina sequencing was performed in duplicate (two runs) and after demultiplexing, the same samples from two independent runs were concatenated.

ORGANISM(S): Sus scrofa  

SUBMITTER: Monika M Kaczmarek   Stefan Bauersachs  Kamil Krawczynski 

PROVIDER: E-GEOD-64863 | ArrayExpress | 2015-11-06

SECONDARY ACCESSION(S): SRP052027GSE64863PRJNA272422

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Expression of microRNAs and isomiRs in the porcine endometrium: implications for gene regulation at the maternal-conceptus interface.

Krawczynski Kamil K   Bauersachs Stefan S   Reliszko Zaneta P ZP   Graf Alexander A   Kaczmarek Monika M MM  

BMC genomics 20151106


Embryo implantation is a complex, synchronized process that requires establishment of a reciprocal dialogue between a receptive endometrium and developing blastocysts. Recently, microRNAs (miRNAs), known to modulate gene expression through post-transcriptional mechanisms, were implicated in regulation of early pregnancy events including maternal recognition of pregnancy and implantation. To characterize complex transcriptomic changes, expression of miRNAs in pregnant and cyclic endometria collec  ...[more]

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