Transcriptomics

Dataset Information

296

RNA-seq Analysis of MoRFX1-dependent Genes in Magnaporthe oryzae 70-15


ABSTRACT: Purpose: The goals of this study are to find out the genes regulated directly or indirectly by the regulatory factor X protein MoRfx1 by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMorfx1 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). Samples were sequenced in a 1 x 100 nt way on Illumina Hiseq2500 instrument using TruSeq PE Cluster Kit v3 - cBot - HS (Illumina) and TruSeq SBS Kit v3-HS (Illumina).The M. oryzae genome database (MG8) (www.broadinstitute.org) was used as a reference gene database. Genome-wide transcript levels of genes were quantified in fragments per kilobase of exon model per million mapped reads (FPKM) (Trapnell 2010). Gene Ontology (GO) enrichment analyses for differentially expressed genes were performed using hypergeometric test with topGO, and p-values were adjusted with Bonferroni for multiple testing (Alexa 2006). And we selected FDR < 0.05 as enrichment terms. Results and Conclusions: We identified 1735 genes regulated by Morfx1 through RNA-seq. Mycelial mRNA profiles of wild type strain 70-15 (WT), ΔMorfx1 incubated in H2O for 4 h were generated, in biologic triplicate, by Illumina Hiseq2500 in a 1 x 100 nt way

ORGANISM(S): Pyricularia oryzae  

SUBMITTER: Jian P Lu   Hui J Cao  Fu C Lin  Jian Ping Lu  Dan D Sun  Peng Y Huang  Li L Zhang 

PROVIDER: E-GEOD-65296 | ArrayExpress | 2016-07-20

SECONDARY ACCESSION(S): SRP052828GSE65296PRJNA273639

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

The regulatory factor X protein MoRfx1 is required for development and pathogenicity in the rice blast fungus Magnaporthe oryzae.

Sun Dandan D   Cao Huijuan H   Shi Yongkai Y   Huang Pengyun P   Dong Bo B   Liu Xiaohong X   Lin Fucheng F   Lu Jianping J  

Molecular plant pathology 20160920 8


Magnaporthe oryzae is a cereal pathogen causing 20%-30% rice yield losses. Regulatory factor X transcription factors are highly conserved proteins with diverse functions among organisms. Here, we show that MoRfx1 is required for cell division, development and pathogenicity in M. oryzae. Deletion of MoRFX1 resulted in reduced growth and conidiation, decreased appressorium turgor and impaired virulence. ΔMorfx1 displayed increased sensitivity to UV light, four DNA-damaging agents and three cell wa  ...[more]

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