Dataset Information


Transient Pairing of Homologous Oct4 Alleles Accompanies the Onset of Embryonic Stem Cell Differentiation

ABSTRACT: The relationship between chromatin organization and transcriptional regulation is an area of intense investigation. We have characterized the spatial relationships between alleles of the Oct4, Sox2, and Nanog genes in single cells during the earliest stages of mouse embryonic stem cell (ESC) differentiation and during embryonic development. We describe homologous pairing of the Oct4 alleles during ESC differentiation and embryogenesis, and present evidence that pairing is correlated with the kinetics of ESC differentiation. Importantly, we identify critical DNA elements within the Oct4 promoter/enhancer region that mediate pairing of Oct4 alleles. Finally, we show that mutation of OCT4/SOX2 binding sites within this region abolishes inter-chromosomal interactions and affects accumulation of the repressive H3K9me2 modification at the Oct4 enhancer. Our findings demonstrate that chromatin organization and transcriptional programs are intimately connected in ESCs, and that the dynamic positioning of the Oct4 alleles is associated with the transition from pluripotency to lineage specification. Examination of chromatin contacts between Oct4 alleles using PE-4Cseq

ORGANISM(S): Mus musculus  

SUBMITTER: David L Spector   Megan S Hogan  Cinthya J Zepeda-Mendoza  Cinthya J Zepeda Mendoza 

PROVIDER: E-GEOD-65510 | ArrayExpress | 2015-02-03



Similar Datasets

2018-11-30 | E-MTAB-5875 | ArrayExpress
2016-07-03 | E-GEOD-64358 | ArrayExpress
2016-07-03 | E-GEOD-64360 | ArrayExpress
2015-01-01 | S-EPMC4355581 | BioStudies
2020-01-01 | S-EPMC7378944 | BioStudies
2020-01-23 | E-MTAB-7816 | ArrayExpress
2013-01-01 | S-EPMC3828132 | BioStudies
2014-05-21 | E-GEOD-50445 | ArrayExpress
2008-07-24 | E-GEOD-11970 | ArrayExpress
2016-08-06 | E-GEOD-71860 | ArrayExpress