Transcriptomics

Dataset Information

6

C-Myb+ erythro-myeloid progenitor-derived fetal monocytes give rise to adult tissue-resident macrophages


ABSTRACT: Although classified as hematopoietic cells, tissue-resident macrophages are selfrenewing and maintained independently of adult hematopoiesis. While most macrophages originate from embryonic precursors that seed tissues prior to birth, their exact origin is unknown. Using an in utero macrophage depletion strategy and fatemapping of yolk sac (YS) and fetal liver (FL) hematopoiesis, we found that YS macrophages are the main precursors of microglia, while most other macrophages derive from fetal monocytes. Both YS macrophages and fetal monocytes arise from erythro-myeloid progenitors (EMP) generated in the YS. In the YS, EMP gave rise to macrophages without monocytic intermediates, while EMP seeding the FL upon the establishment of blood circulation acquired c-Myb expression and gave rise to fetal monocytes that then seed embryonic tissues to differentiate into macrophages. Thus, adult tissue-resident macrophages established from HSC-independent embryonic precursors arise from two different developmental programs. 12 samples of progenitors, monocytes or macrophages are analyzed from 2 to 4 replicate. Each replicate derived from at least 5 embryos or adult mice

ORGANISM(S): Mus musculus  

SUBMITTER: Yonit Lavin   Francisca F Almeida  Peter See  Michael Poidinger  Burkhard Becher  Camilla Forsberg  Jinmiao Chen  Florent Ginhoux  Guillaume Hoeffel  Donovan Low  jinmiao chen  Igor M Samokhvalov  Anna E Beaudin  Anis Larbi  Melanie Greter  Lai G Ng  Ivy Low  Josephine Lum  Francesca Zolezzi  Miriam Merad 

PROVIDER: E-GEOD-66970 | ArrayExpress | 2015-04-23

SECONDARY ACCESSION(S): GSE66970PRJNA278517

REPOSITORIES: GEO, ArrayExpress

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Publications


Although classified as hematopoietic cells, tissue-resident macrophages (MFs) arise from embryonic precursors that seed the tissues prior to birth to generate a self-renewing population, which is maintained independently of adult hematopoiesis. Here we reveal the identity of these embryonic precursors using an in utero MF-depletion strategy and fate-mapping of yolk sac (YS) and fetal liver (FL) hematopoiesis. We show that YS MFs are the main precursors of microglia, while most other MFs derive f  ...[more]

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