Transcriptomics

Dataset Information

300

Transcriptome analysis of poplar during leaf spot infection with Sphaerulina spp.


ABSTRACT: Diseases of poplar caused by the fungal pathogen Sphaerulina musiva and related species are of growing concern, particular with the increasing interest in intensive popluliculture to meet increasing energy demands. S. musiva is able to cause infection on leaves, resulting in defoliation and canker formation on stems. To gain a greater understanding of the different responses of poplar species to infection with their natural Sphaerulina species, RNA-seq was conducted on leaves of Populus deltoides, P. balsamifera and P. tremuloides infected with S. musiva, S. populicola and a new undescribed species Ston1, respectively. Progression of disease symptoms, pathogen growth and host response were detected. Through the time course of infection, different and species-specific metabolic pathways were activated. In all three species, genes associated with growth and development were down-regulated, while genes involved the phenylpropanoid, terpenoid and flavonoid biosynthesis were up-regulated. Poplar defensive genes were expressed early in P. balsamifera and P. tremuloides, but delayed in P. deltoides, which correlated with the rate of disease symptoms development. This data gives an insight into the large differences in timing and expression of genes between poplar species being attacked with their native associated Sphaerulina pathogen. RNA-seq was conducted on leaves of Populus deltoides, P. balsamifera and P. tremuloides infected with S. musiva, S. populicola and a new undescribed species Ston1, respectively.

SUBMITTER: Armand Séguin   Adam Foster  Philippe Tanguay  Adam J Foster  Gervais Pelletier 

PROVIDER: E-GEOD-67697 | ArrayExpress | 2015-08-28

SECONDARY ACCESSION(S): GSE67697SRP057004PRJNA280709

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Transcriptome Analysis of Poplar during Leaf Spot Infection with Sphaerulina spp.

Foster Adam J AJ   Pelletier Gervais G   Tanguay Philippe P   Séguin Armand A  

PloS one 20150917 9


Diseases of poplar caused by the native fungal pathogen Sphaerulina musiva and related species are of growing concern, particularly with the increasing interest in intensive poplar plantations to meet growing energy demands. Sphaerulina musiva is able to cause infection on leaves, resulting in defoliation and canker formation on stems. To gain a greater understanding of the different responses of poplar species to infection caused by the naturally co-evolved Sphaerulina species, RNA-seq was cond  ...[more]

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