Transcriptomics

Dataset Information

298

Next Generation Sequencing Facilitates Small RNA Mediated Rice Resistance to Xoo


ABSTRACT: Purpose: The goal of this study is to identify small non-conding RNAs which are involved in rice resistance to Xoo. Methods: Rice leaves were inoculated with the Xoo strain PXO61 at the four-leaf to five-leaf stage by the leaf-clipping method. Control rice plants were inoculated with water (mock inoculation). And then, total RNA was extracted to be sequenced using Illumina GAIIx. Results: Using an optimized data analysis workflow to count the expression level of small ncRNA, we found several differentially expressed small ncRNA which may be participated in the interaction between rice and Xoo. Conclusions: Small ncRNA have be found to function in a variety of biological processes. Our study here has showed that several candidate miRNA or siRNA may play a significant role in rice immunity. Plants were inoculated with the Xoo strain PXO61 at the four-leaf to five-leaf stage by the leaf-clipping method. Control rice plants were inoculated with water (mock inoculation). Samples were collected before inoculation (ck) and at 2, 4, and 24 hours after PXO61 or mock inoculation from Rb49 and MDJ8. Leaf fragments approximately 2 cm in length that were immediately next to the inoculation site were collected.

ORGANISM(S): Oryza sativa Japonica Group  

SUBMITTER: Xianghua Li   hanming hong  Shiping Wang  Yanyan Liu  Jinghua Xiao  Hanming Hong  Haitao Zhang 

PROVIDER: E-GEOD-69242 | ArrayExpress | 2016-02-25

SECONDARY ACCESSION(S): SRP058724GSE69242PRJNA284956

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Small RNAs and Gene Network in a Durable Disease Resistance Gene--Mediated Defense Responses in Rice.

Hong Hanming H   Liu Yanyan Y   Zhang Haitao H   Xiao Jinghua J   Li Xianghua X   Wang Shiping S  

PloS one 20150903 9


Accumulating data have suggested that small RNAs (sRNAs) have important functions in plant responses to pathogen invasion. However, it is largely unknown whether and how sRNAs are involved in the regulation of rice responses to the invasion of Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight, the most devastating bacterial disease of rice worldwide. We performed simultaneous genome-wide analyses of the expression of sRNAs and genes during early defense responses of rice to Xoo  ...[more]

Similar Datasets

| GSE69235 | GEO
2012-03-12 | E-MEXP-3388 | ArrayExpress
2012-02-27 | E-GEOD-36093 | ArrayExpress
2015-05-15 | E-GEOD-63337 | ArrayExpress
2009-12-24 | E-GEOD-8216 | ArrayExpress
2012-10-15 | E-GEOD-34192 | ArrayExpress
2010-12-01 | GSE19844 | GEO
2010-12-01 | E-GEOD-19844 | ArrayExpress
2012-12-25 | E-GEOD-41798 | ArrayExpress
2008-06-14 | E-GEOD-6244 | ArrayExpress