Transcriptomics

Dataset Information

297

Transcription profiling by high throughput sequencing of Arabidopsis fip37-4 LEC1:FIP37 seedlings


ABSTRACT: N6-methyladenosine (m6A) represents the most prevalent internal modification on messenger RNA, and requires a multicomponent m6A methyltransferase complex in mammals. How their plant counterparts determine the global m6A modification landscape and its molecular link to plant development remain elusive. Here we show that FKBP12 INTERACTING PROTEIN 37 KD (FIP37) is a core component of the m6A methyltransferase complex, which underlies control of shoot stem cell fate in Arabidopsis. The mutants lacking FIP37 exhibit massive overproliferation of shoot meristems and a transcriptome-wide loss of m6A RNA modifications. We further demonstrate that FIP37 mediates m6A RNA modification on key shoot meristem genes inversely correlated with their mRNA stability, thus confining their transcript levels to prevent shoot meristem overproliferation. Our results suggest an indispensable role of FIP37 in mediating m6A mRNA modification, which is required for maintaining the shoot meristem as a renewable source for continuously producing all aerial organs in plants. RNA-seq in Arabidopsis thaliana (Col-0) wild-type and fip37-4 LEC1:FIP37, three replicates for each sample

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Hao Yu   Lu Liu  Lisha Shen  Xiaofeng Gu  Zhiwei N Teo  Weiling M Cai  Ying Chen  Zhe Liang  Peter C Dedon  Xingliang Hou 

PROVIDER: E-GEOD-75507 | ArrayExpress | 2016-07-25

SECONDARY ACCESSION(S): SRP066805GSE75507PRJNA304437

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

N(6)-Methyladenosine RNA Modification Regulates Shoot Stem Cell Fate in Arabidopsis.

Shen Lisha L   Liang Zhe Z   Gu Xiaofeng X   Chen Ying Y   Teo Zhi Wei Norman ZW   Hou Xingliang X   Cai Weiling Maggie WM   Dedon Peter C PC   Liu Lu L   Yu Hao H  

Developmental cell 20160707 2


N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification on mRNA and requires a multicomponent m(6)A methyltransferase complex in mammals. How their plant counterparts determine the global m(6)A modification landscape and its molecular link to plant development remain unknown. Here we show that FKBP12 INTERACTING PROTEIN 37 KD (FIP37) is a core component of the m(6)A methyltransferase complex, which underlies control of shoot stem cell fate in Arabidopsis. The mutants lac  ...[more]

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