Transcriptomics

Dataset Information

78

SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing [CLIP-Seq]


ABSTRACT: SIRT7 is an NAD+-dependent protein deacetylase with important roles in ribosome biogenesis and cell proliferation. Previous studies have established that SIRT7 is associated with RNA polymerase I, interacts with pre-rRNA and promotes rRNA synthesis. Here we show that SIRT7 is also associated with snoRNAs that are involved in pre-rRNA processing and rRNA maturation. Knockdown of SIRT7 impairs U3 snoRNA-dependent early cleavage steps that are necessary for generation of 18S rRNA. Mechanistically, SIRT7 deacetylates U3-55k, a core component of the U3 snoRNP complex, and reversible acetylation of U3-55k modulates the association of U3-55k with U3 snoRNA. Deacetylation by SIRT7 enhances U3-55k binding to U3 snoRNA, which is a prerequisite for pre-rRNA processing. Under stress conditions, SIRT7 is released from nucleoli, leading to hyperacetylation of U3-55k and attenuation of prerRNA processing. The results reveal a multifaceted role of SIRT7 in ribosome biogenesis, regulating both transcription and processing of rRNA. CLIP-seq was performed in Flag-SIRT7-293T cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: Ingrid Grummt  Renate Voit   Aishwarya Iyer   Maximilian F Blank   Bingding Huang   Lin Wang   Sifan Chen    

PROVIDER: E-GEOD-76578 | ArrayExpress| 2016-01-17

SECONDARY ACCESSION(S): GSE76578SRP068144PRJNA308081

REPOSITORIES: GEO, ArrayExpress, ENA

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