Transcriptomics

Dataset Information

6

Transcription profiling of mouse ES cells and EpiSCs


ABSTRACT: The application of human embryonic stem (ES) cells in medicine; and biology has an inherent reliance on understanding the starting; cell population. Human ES cells differ from mouse ES cells and the; specific embryonic origin of both cell types is unclear. Previous; work suggested that mouse ES cells could only be obtained from; the embryo before implantation in the uterus1–5. Here we show; that cell lines can be derived from the epiblast, a tissue of the postimplantation; embryo that generates the embryo proper. These; cells, which we refer to as EpiSCs (post-implantation epiblastderived; ES cells), express transcription factors known to regulate; pluripotency, maintain their genomic integrity, and robustly differentiate; into the major somatic cell types as well as primordial; germ cells. The EpiSC lines are distinct from mouse ES cells in; their epigenetic state and the signals controlling their differentiation. Furthermore, EpiSC and human ES cells share patterns of; gene expression and signalling responses that normally function; in the epiblast. These results show that epiblast cells can be maintained; as stable cell lines and interrogated to understand how; pluripotent cells generate distinct fates during early development. *Note: EpiSCs were previously referred to as post-ES cells Experiment Overall Design: 3 biological replicates of each cell type (EpiSC and mouse ES) were compared.

ORGANISM(S): Mus musculus  

SUBMITTER: Paul Tesar 

PROVIDER: E-GEOD-7866 | ArrayExpress | 2008-06-19

SECONDARY ACCESSION(S): GSE7866PRJNA105501

REPOSITORIES: GEO, ArrayExpress

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Publications

New cell lines from mouse epiblast share defining features with human embryonic stem cells.

Tesar Paul J PJ   Chenoweth Josh G JG   Brook Frances A FA   Davies Timothy J TJ   Evans Edward P EP   Mack David L DL   Gardner Richard L RL   McKay Ronald D G RD  

Nature 20070627 7150


The application of human embryonic stem (ES) cells in medicine and biology has an inherent reliance on understanding the starting cell population. Human ES cells differ from mouse ES cells and the specific embryonic origin of both cell types is unclear. Previous work suggested that mouse ES cells could only be obtained from the embryo before implantation in the uterus. Here we show that cell lines can be derived from the epiblast, a tissue of the post-implantation embryo that generates the embry  ...[more]

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