Transcriptomics

Dataset Information

337

Global transcriptome analysis in the MYCN-amplified neuroblastoma cell line IMR5-75 upon inducible MYCN-knockdown


ABSTRACT: Inducible MYCN-knockdown, followed by RNA-seq analysis in the MYCN-amplified neuroblastoma cell line IMR5-75, reveals profound time-dependent transcriptome changes. For modulation of MYCN levels, stable neuroblastoma cell models were used where MYCN can be downregulated via vector-based hairpin RNA induction upon addition of 1µg/ml tetracycline (IMR5-75-shMYCN. From cells treated either with tetracycline or solvent (ethanol), RNA was isolated at time points 6 hours, 12 hours and 24 hours. Experiments were done in duplicates. RNA was sequenced.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kai O Henrich   David J Duffy  Christoph Plass  Peter Schmezer  Thomas Höfer  Martha Parzonka  Daniel Dreidax  Sebastian Bender  Moritz Gartlgruber  Stefan M Pfister  Axel Benner  Maral Saadati  Chunxuan Shao  Frank Westermann  Matthias Fischer  Carl Herrmann 

PROVIDER: E-GEOD-80397 | ArrayExpress | 2016-09-08

SECONDARY ACCESSION(S): GSE80397SRP073460PRJNA318814

REPOSITORIES: GEO, ArrayExpress, ENA

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