Transcriptomics

Dataset Information

297

The Zinc Finger Transcription Factor Sp9 Is Required for the Development of Striatopallidal Projection Neurons


ABSTRACT: Purpose: To assess changes in gene expression profiles from the P0 littermate Sp9Flox/Flox control striatum (including SVZ) and conditional mutant striatum of Drd2-Cre; Sp9Flox/Flox mice. Methods: total RNA was isolated and sequenced from the striatum of the P0 littermate Sp9Flox/Flox control or conditional mutant striatum of Drd2-Cre; Sp9Flox/Flox mice in triplicate using an Illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which demonstrated fold-change>=2, and p value <=0.05. Changed genes were then filtered to reveal Sp9 downstream targets which were altered in vivo due to the loss of Sp9 expression. Results: 179 genes were significantly increased and 147 genes were significantly decreased in expression level due to the loss of Sp9 expression. total RNA was isolated and sequenced from the striatum of the P0 littermate Sp9Flox/Flox control or conditional mutant striatum of Drd2-Cre; Sp9Flox/Flox mice in triplicate using an Illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which demonstrated fold-change>=2, and p value <=0.05. Changed genes were then filtered to reveal Sp9 downstream targets which were altered in vivo due to the loss of Sp9 expression.

ORGANISM(S): Mus musculus  

SUBMITTER: C Wang   L An  Z Xiong  Q Zhang  Z Xu  Zhengang Yang  Y You  B Chen  J L Rubenstein  Y Zhang  J Li  Z Liu  M He  Q Liang 

PROVIDER: E-GEOD-83373 | ArrayExpress | 2016-06-16

SECONDARY ACCESSION(S): SRP076589GSE83373PRJNA325728

REPOSITORIES: GEO, ArrayExpress, ENA

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