Transcriptomics

Dataset Information

297

RNA-Seq analysis of wild-type and Brpf1-null fetal liver LSK cells


ABSTRACT: To compare the impact of hematopoietic-specific Brpf1 gene inactivation, LSK (Lin-Sca1+cKit1+) cells were sorted from wild-type and Brpf1-null fetal liver cells for RNA-Seq. Four E14.5 embryos were used to pool sufficient LSK cells for total RNA isolation and subsequent sequencing on HiSq2500. Two independent pairs of wild-type and mutant RNA samples (each of which contained LSK cells pooled from four embryos) were used for oligo-dT primed RNA Seq.

ORGANISM(S): Mus musculus  

SUBMITTER: Xiang-Jiao Yang   Linya You  Edwin Wang  Jinfeng Zou 

PROVIDER: E-GEOD-83483 | ArrayExpress | 2016-08-10

SECONDARY ACCESSION(S): SRP076721GSE83483PRJNA326114

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

BRPF1 is essential for development of fetal hematopoietic stem cells.

You Linya L   Li Lin L   Zou Jinfeng J   Yan Kezhi K   Belle Jad J   Nijnik Anastasia A   Wang Edwin E   Yang Xiang-Jiao XJ  

The Journal of clinical investigation 20160808 9


Hematopoietic stem cells (HSCs) serve as a life-long reservoir for all blood cell types and are clinically useful for a variety of HSC transplantation-based therapies. Understanding the role of chromatin organization and regulation in HSC homeostasis may provide important insights into HSC development. Bromodomain- and PHD finger-containing protein 1 (BRPF1) is a multivalent chromatin regulator that possesses 4 nucleosome-binding domains and activates 3 lysine acetyltransferases (KAT6A, KAT6B, a  ...[more]

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