Transcriptomics

Dataset Information

4

Gene Expression Profiling of Single Embryos, Significant Effects of In Vitro Maturation, Fertilization and Culture


ABSTRACT: In vitro production (IVP) has been shown to affect embryonic gene expression and often result in large offspring syndrome (LOS) in cattle and sheep. To dissect the effects of in vitro maturation, fertilization and culture, we compared the expression profiles of single bovine blastocysts generated by: 1) in vitro maturation, fertilization and culture (IVF); 2) in vivo maturation, fertilization and in vitro culture (IVD); and 3) in vivo maturation, fertilization and development (AI). To conduct expression profiling, total RNA was isolated from individual embryos, linearly amplified and hybridized to a custom bovine cDNA microarray containing approximately 6,300 unique genes. There were 306, 367 and 200 genes differentially expressed between the AI and IVD, IVF and IVD and AI and IVF comparisons, respectively. Interestingly, 44 differentially expressed genes were identified between the AI embryos and both the IVF and IVD embryos, making these potential candidates for LOS. There were 60 genes differentially expressed between the IVF embryos and the AI and IVD embryos. The Gene Ontology category “RNA processing” was over-represented among the genes that were down-regulated in the IVF embryos, indicating an effect of in vitro oocyte maturation/fertilization on embryonic gene expression. A culture effect on the expression of genes involved in translation was also observed by the comparison of AI to IVD embryos. Reference design with dye swap. 43 individual embryos were analyzed.

ORGANISM(S): Bos taurus  

SUBMITTER: Tshimangadzo L Nedambale   Robin E Everts  Li-Ying Sung  Sandra L Rodriguez-Zas  Xiuchun Tian  Raymond L Page  Harris A Lewin  Sadie L Smith  Xiangzhong Yang  Jean-Paul Renard  Boyd Henderson  Fuliang Du 

PROVIDER: E-GEOD-8807 | ArrayExpress | 2008-05-04

SECONDARY ACCESSION(S): GSE8807PRJNA102119

REPOSITORIES: GEO, ArrayExpress

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Publications

Gene expression profiling of single bovine embryos uncovers significant effects of in vitro maturation, fertilization and culture.

Smith Sadie L SL   Everts Robin E RE   Sung Li-Ying LY   Du Fuliang F   Page Raymond L RL   Henderson Boyd B   Rodriguez-Zas Sandra L SL   Nedambale Tshimangadzo L TL   Renard Jean-Paul JP   Lewin Harris A HA   Yang Xiangzhong X   Tian X Cindy XC  

Molecular reproduction and development 20090101 1


In vitro production (IVP) has been shown to affect embryonic gene expression and often result in large offspring syndrome (LOS) in cattle and sheep. To dissect the effects of in vitro maturation, fertilization and culture on bovine embryos, we compared the expression profiles of single blastocysts generated by: (1) in vitro maturation, fertilization and culture (IVF); (2) in vivo maturation, fertilization and in vitro culture (IVD); and (3) in vivo maturation, fertilization and development (AI).  ...[more]

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