Transcriptomics

Dataset Information

7

Progression of heart failure induced by tachycardia


ABSTRACT: Analysis of changes of gene expression profiles in the left ventricle (LV) during the progression of heart failure (HF) in the canine tachypacing induced HF model. Gene expression profiling was performed on samples collected at different time points representing various stages of LV dysfunction, i.e. tachypaced for 3 days (Day-3), 1 week (Week-1), 2 weeks (Week-2), 3-4 weeks (End stage), and unpaced controls (Day-0). Results provide insights into molecular mechanisms of heart failure progression. Keywords: time course This work aims to study changes of gene expression profiles in the left ventricle (LV) during the progression of heart failure (HF) in the canine tachypacing induced HF model. LV transmural tissue sections were collected at different time points after initiating rapid pacing representing various stages of LV dysfunction, i.e. tachypaced for 3 days (Day-3), 1 week (Week-1), 2 weeks (Week-2), 3-4 weeks (End stage), and unpaced controls (Day-0). As biological replicates, tissues were collected separately from 3 individual dogs at each of the time points. In total we collected 15 tissue samples for all 5 time points. Three RNA samples were independently isolated from each of the 15 tissue samples as technical replicates. The resulting 45 RNA samples were used for microarray experiment. Affymetrix gene expression profiles of the 45 RNA samples were used for all data analysis in this study.

ORGANISM(S): Canis lupus familiaris  

SUBMITTER: David A Kass  Raimond L Winslow  Fadi G Akar  Zhong Gao  Gordon F Tomaselli  Antti Tanskanen  Deborah DiSilvestre  Yanli Tian 

PROVIDER: E-GEOD-9794 | ArrayExpress | 2010-05-19

SECONDARY ACCESSION(S): GSE9794PRJNA103733

REPOSITORIES: GEO, ArrayExpress

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Publications

Key pathways associated with heart failure development revealed by gene networks correlated with cardiac remodeling.

Gao Zhong Z   Barth Andreas S AS   DiSilvestre Deborah D   Akar Fadi G FG   Tian Yanli Y   Tanskanen Antti A   Kass David A DA   Winslow Raimond L RL   Tomaselli Gordon F GF  

Physiological genomics 20080909 3


Heart failure (HF) is the leading cause of morbidity and mortality in the industrialized world. While the transcriptomic changes in end-stage failing myocardium have received much attention, no information is available on the gene expression patterns associated with the development of HF in large mammals. Therefore, we used a well-controlled canine model of tachycardia-induced HF to examine global gene expression in left ventricular myocardium with Affymetrix canine oligonucleotide arrays at var  ...[more]

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