Transcriptomics

Dataset Information

8

Transcription profiling of Bos taurus immature oocytes and 8-cell embryos to assess the impact of the loss of sample representation caused by the use of global linear amplification (T7-IVT) during microarray hybridization sample preparation


ABSTRACT: Assessing the impact of the loss of sample representation caused by the use of global linear amplification (T7-IVT) during microarray hybridization sample preparation.

ORGANISM(S): Bos taurus  

SUBMITTER: Isabelle Gilbert  

PROVIDER: E-MEXP-2099 | ArrayExpress | 2009-03-25

REPOSITORIES: ArrayExpress

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Publications

Real-time monitoring of aRNA production during T7 amplification to prevent the loss of sample representation during microarray hybridization sample preparation.

Gilbert Isabelle I   Scantland Sara S   Dufort Isabelle I   Gordynska Olga O   Labbe Aurélie A   Sirard Marc-André MA   Robert Claude C  

Nucleic acids research 20090330 8


Gene expression analysis performed through comparative abundance of transcripts is facing a new challenge with the increasing need to compare samples of known cell number, such as early embryos or laser microbiopsies, where the RNA contents of identical cellular inputs can by nature be variable. When working with scarce tissues, the success of microarray profiling largely depends on the efficiency of the amplification step as determined by its ability to preserve the relative abundance of transc  ...[more]

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