Transcriptomics

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Transcription profiling by array of human MOLT4 cells after gamma irradiation and treatment with (E)3-[(4-Methylphenyl)sulfonyl]-2-propenenitrile


ABSTRACT: MOLT4 cells in log phase (1x106 cells/ml) were pre-treated for 2 hours with the I?B? phosphorylation inhibitor BAY 11-7082 (Calbiochem) at 5?M, and the control samples with corresponding volume of DMSO. Cells were then irradiated with 4 Gy at room temperature, at a dose rate of 2.45 Gy/minute with a 137Cs ?-irradiator. Cells were harvested 2 hours after irradiation and RNA was extracted (Trizol; Invitrogen). RNA and cRNA quantity and quality was determined by Nanodrop spectrophotometer and Bioanalyser 2100 (Agilent). Affymetrix GeneChip® Human Genome U133A 2.0 arrays were hybridized as standard (www.affymetrix.co.uk). Array quality was determined using R and Affymetrix Expression Console file values. We then calculated a Z-score which represents the degree to which BAY 11-7082 reduces irradiation-induced transcription of all target genes.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Efterpi Papouli  

PROVIDER: E-MEXP-2177 | ArrayExpress | 2009-06-30

REPOSITORIES: ArrayExpress

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