Dataset Information


Transcription profiling by array of C. albicans to investigate antifungal resistance

ABSTRACT: A pre-culture in NGY (overnight 30°C) was used to inoculate RPMI-1640 medium to an initial optical density at 600 nm (OD600) of 0.05. Cells were then grown to mid exponential phase (OD600 = 0.50.6) at the appropriate temperature (25°C or 35°C) before the culture was

split into two aliquots and fluconazole (32 ?g ml1 final concentration) added to one; the other serving as the control. After 2 h the cells were harvested by centrifugation and flash

frozen in liquid nitrogen. Three independent cultures were grown for RNA extraction for each isolate at each condition. RNA was prepared from cell pellets with TRIZOL® reagent (Invitrogen) and a mechanical disruption method.

ORGANISM(S): Candida albicans  


PROVIDER: E-MEXP-3000 | ArrayExpress | 2014-05-02


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