Transcriptomics

Dataset Information

122

Effects of SocB toxin on genome-wide expression in Caulobacter crescentus


ABSTRACT: Caulobacter cells were grown to exponential phase in rich PYE medium. At time zero, expression of socB-M2 was induced by the addition of 0.3% xylose. A reference sample was also collected at this time point. Atter 1.5 hours, the experimental sample was then collected. RNA was extracted from the reference and experimental samples, reverse transcribed into Cy3- or Cy5-labeled cDNA, and then hybridized to Agilent 4x44K Caulobacter arrays.

ORGANISM(S): Caulobacter vibrioides  

SUBMITTER: Christopher Aakre  

PROVIDER: E-MEXP-3990 | ArrayExpress | 2013-12-10

REPOSITORIES: ArrayExpress

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Publications

A bacterial toxin inhibits DNA replication elongation through a direct interaction with the β sliding clamp.

Aakre Christopher D CD   Phung Tuyen N TN   Huang David D   Laub Michael T MT  

Molecular cell 20131114 5


Toxin-antitoxin (TA) systems are ubiquitous on bacterial chromosomes, yet the mechanisms regulating their activity and the molecular targets of toxins remain incompletely defined. Here, we identify SocAB, an atypical TA system in Caulobacter crescentus. Unlike canonical TA systems, the toxin SocB is unstable and constitutively degraded by the protease ClpXP; this degradation requires the antitoxin, SocA, as a proteolytic adaptor. We find that the toxin, SocB, blocks replication elongation throug  ...[more]

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