Transcriptomics

Dataset Information

9

Transcription profiling of Ectocarpus strain Esil32 porophyte and gametophyte stages in normal and mutant strains to analyse expression of two sets of genes previously identified as being putatively sporophyte-specific or gametophyte-specific


ABSTRACT: The aim of this experiment was to analyse the expression of two sets of genes identified as being putatively sporophyte-specific or gametophyte-specific by a suppressive subtraction hybridisation using cDNA from immature sporophytes and immature gametophytes of the Ectocarpus strain Esil32. The expression of these genes was analysed in the sporophyte and gametophyte generations of the life cycle (again using immature algae that had not yet produces zoidangia) and in the sporophyte generation of a mutant strain, immediate upright, that exhibits gametophyte-like characteristics during the sporophyte generation.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Ectocarpus siliculosus  

SUBMITTER: Delphine Scornet   J. Mark Cock  Akira F Peters  Morgane Ratin 

PROVIDER: E-MEXP-848 | ArrayExpress | 2007-08-01

REPOSITORIES: ArrayExpress

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Publications

Life-cycle-generation-specific developmental processes are modified in the immediate upright mutant of the brown alga Ectocarpus siliculosus.

Peters Akira F AF   Scornet Delphine D   Ratin Morgane M   Charrier Bénédicte B   Monnier Annabelle A   Merrien Yves Y   Corre Erwan E   Coelho Susana M SM   Cock J Mark JM  

Development (Cambridge, England) 20080313 8


Development of the sporophyte and gametophyte generations of the brown alga E. siliculosus involves two different patterns of early development, which begin with either a symmetric or an asymmetric division of the initial cell, respectively. A mutant, immediate upright (imm), was isolated that exhibited several characteristics typical of the gametophyte during the early development of the sporophyte generation. Genetic analyses showed that imm is a recessive, single-locus Mendelian factor and an  ...[more]

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