Transcriptomics

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Transcription profiling by array of epididymal adipose tissue from male hemizygous fat-1(+/-) transgenic mice and their wild-type littermates, all fed with a high-fat diet


ABSTRACT: Dietary administration of n-3 polyunsaturated fatty acids (PUFA) is often associated with altered adipose tissue (AT) morphology and/or function in obese mice. However, it is unclear whether these observations are an indirect consequence of reduced weight gain or result from direct actions of n-3 PUFA. Here we studied the AT of fat-1 transgenic mice that convert endogenous n-6 to n-3 PUFA. These mice display equivalent weight gain and fat accretion to their wild-type (WT) counterparts. We performed Affymetrix microarray in epididymal AT of male hemizygous fat-1(+/-) transgenic mice and their wild type littermates that had been fed high fat diets from 6 weeks of age (diet-93075, 55% Kcal from fat, Harlan Teklad). Mice were sacrificed after 8 weeks on the HF diet. At sacrifice epididymal adipose tissues excised for the microarray study (n=3 per group) were rapidly homogenized in QIAZOL (QIAGEN) and snap frozen in liquid nitrogen.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Phillip J White   Andre Marette  

PROVIDER: E-MTAB-1831 | ArrayExpress | 2015-02-06

REPOSITORIES: ArrayExpress

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