Transcriptomics

Dataset Information

298

RNA-seq of cellular mRNAs from protrusions and cell-body of MDA-MB-231 breast carcinoma cells


ABSTRACT: We quantified the distribution of cellular mRNAs between protrusions and cell-body of MDA-MB-231 breast carcinoma cells by RNA-seq, using a filter based fractionation method. In this method, cells are seeded on top of 3micrometer transwell filters to enable protrusions to form but to prevent the cell-bodies passing through due to the small size of the pores, thus resulting in separation of protrusions and cell-bodies on opposite sides of the filter, from which RNA can be prepared separately. RNA samples for Illumina sequencing were generated in duplicates from two biological replicate experiments (two protrusions and two cell-body).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens  

SUBMITTER: Faraz K Mardakheh  

PROVIDER: E-MTAB-2546 | ArrayExpress | 2015-11-08

SECONDARY ACCESSION(S): ERP005736

REPOSITORIES: ArrayExpress, ENA

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Publications

Global Analysis of mRNA, Translation, and Protein Localization: Local Translation Is a Key Regulator of Cell Protrusions.

Mardakheh Faraz K FK   Paul Angela A   Kümper Sandra S   Sadok Amine A   Paterson Hugh H   Mccarthy Afshan A   Yuan Yinyin Y   Marshall Christopher J CJ  

Developmental cell 20151101 3


Polarization of cells into a protrusive front and a retracting cell body is the hallmark of mesenchymal-like cell migration. Many mRNAs are localized to protrusions, but it is unclear to what degree mRNA localization contributes toward protrusion formation. We performed global quantitative analysis of the distributions of mRNAs, proteins, and translation rates between protrusions and the cell body by RNA sequencing (RNA-seq) and quantitative proteomics. Our results reveal local translation as a  ...[more]

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