Transcriptomics

Dataset Information

64

Transcription profiling by array of human H9 embryonic stem cells (wild type and BRACHYURY shRNA knockdown) differentiated for 36h or 72h in chemically-defined culture media


ABSTRACT: Transcriptional profiling of H9 hESCs (wild type and BRACHYURY shRNA knockdown) differentiated for 36h or 72h in chemically-defined culture media supplemented with FGF2, LY294002 and either BMP4 or ActivinA.

ORGANISM(S): Homo sapiens  

SUBMITTER: Tiago Faial  

PROVIDER: E-MTAB-2912 | ArrayExpress | 2015-05-19

REPOSITORIES: ArrayExpress

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Publications

Brachyury and SMAD signalling collaboratively orchestrate distinct mesoderm and endoderm gene regulatory networks in differentiating human embryonic stem cells.

Faial Tiago T   Bernardo Andreia S AS   Mendjan Sasha S   Diamanti Evangelia E   Ortmann Daniel D   Gentsch George E GE   Mascetti Victoria L VL   Trotter Matthew W B MW   Smith James C JC   Pedersen Roger A RA  

Development (Cambridge, England) 20150526 12


The transcription factor brachyury (T, BRA) is one of the first markers of gastrulation and lineage specification in vertebrates. Despite its wide use and importance in stem cell and developmental biology, its functional genomic targets in human cells are largely unknown. Here, we use differentiating human embryonic stem cells to study the role of BRA in activin A-induced endoderm and BMP4-induced mesoderm progenitors. We show that BRA has distinct genome-wide binding landscapes in these two cel  ...[more]

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