Genomics,Multiomics

Dataset Information

90

ChIPseq profile of RNA polymerase (pol II), the p65 subunit of NFkB, STAT1 and STAT2 in bone marrow derived macrophages after treatment with IFNb, heat-killed Listeria monocytogenes (hkL), or both


ABSTRACT: Using ChIP-Seq analysis we define genes induced in macrophages through synergistic transcriptional activation by NFkB and the IFNb-activated transcription factor ISGF3. Microarray expression profiling data from the same study has also been deposited at ArrayExpress under accession number E-MTAB-3015 (https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3015/).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Andrea Majoros  Mathias Mueller  Priyank Shukla  Sebastian Wienerroither  Thomas Decker           

PROVIDER: E-MTAB-2972 | ArrayExpress| 2016-08-22

SECONDARY ACCESSION(S): ERP007140

REPOSITORIES: ArrayExpress, ENA

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Publications

Response to interferons and antibacterial innate immunity in the absence of tyrosine-phosphorylated STAT1.

Majoros Andrea A   Platanitis Ekaterini E   Szappanos Daniel D   Cheon HyeonJoo H   Vogl Claus C   Shukla Priyank P   Stark George R GR   Sexl Veronika V   Schreiber Robert R   Schindler Christian C   Müller Mathias M   Decker Thomas T  

EMBO reports 20160212 3


Signal transducer and activator of transcription 1 (STAT1) plays a pivotal role in the innate immune system by directing the transcriptional response to interferons (IFNs). STAT1 is activated by Janus kinase (JAK)-mediated phosphorylation of Y701. To determine whether STAT1 contributes to cellular responses without this phosphorylation event, we generated mice with Y701 mutated to a phenylalanine (Stat1(Y701F)). We show that heterozygous mice do not exhibit a dominant-negative phenotype. Homozyg  ...[more]

Publication: 1/2

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