Dataset Information


Pancreatic beta cell identity is maintained by DNA methylation-mediated repression of Arx

ABSTRACT: Methylated DNA Immunoprecipitation (meDIP) was used to pull down regions of methylated genomic DNA from beta and alpha cell lines (MIN6 and alpha-TC1, respectively). Agilent promoter tiling array was used to look for regions of differential methylation around key endocrine cell fate determination genes.

INSTRUMENT(S): Model-G2505B US23502366

ORGANISM(S): Mus musculus  

SUBMITTER: Senta Georgia   Anil Bhushan  

PROVIDER: E-MTAB-3243 | ArrayExpress | 2014-12-24


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Pancreatic β cell identity is maintained by DNA methylation-mediated repression of Arx.

Dhawan Sangeeta S   Georgia Senta S   Tschen Shuen-Ing SI   Fan Guoping G   Bhushan Anil A  

Developmental cell 20110401 4

Adult pancreatic β cells can replicate during growth and after injury to maintain glucose homeostasis. Here, we report that β cells deficient in Dnmt1, an enzyme that propagates DNA methylation patterns during cell division, were converted to α cells. We identified the lineage determination gene aristaless-related homeobox (Arx), as methylated and repressed in β cells, and hypomethylated and expressed in α cells and Dnmt1-deficient β cells. We show that the methylated region of the Arx locus in  ...[more]

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