Transcriptomics

Dataset Information

32

Pancreatic beta cell identity is maintained by DNA methylation-mediated repression of Arx


ABSTRACT: Methylated DNA Immunoprecipitation (meDIP) was used to pull down regions of methylated genomic DNA from beta and alpha cell lines (MIN6 and alpha-TC1, respectively). Agilent promoter tiling array was used to look for regions of differential methylation around key endocrine cell fate determination genes.

INSTRUMENT(S): Model-G2505B US23502366

ORGANISM(S): Mus musculus  

SUBMITTER: Senta Georgia  Anil Bhushan     

PROVIDER: E-MTAB-3243 | ArrayExpress| 2015-01-21

REPOSITORIES: ArrayExpress

altmetric image

Publications

Pancreatic β cell identity is maintained by DNA methylation-mediated repression of Arx.

Dhawan Sangeeta S   Georgia Senta S   Tschen Shuen-Ing SI   Fan Guoping G   Bhushan Anil A  

Developmental cell 20110401 4


Adult pancreatic β cells can replicate during growth and after injury to maintain glucose homeostasis. Here, we report that β cells deficient in Dnmt1, an enzyme that propagates DNA methylation patterns during cell division, were converted to α cells. We identified the lineage determination gene aristaless-related homeobox (Arx), as methylated and repressed in β cells, and hypomethylated and expressed in α cells and Dnmt1-deficient β cells. We show that the methylated region of the Arx locus in  ...[more]

Similar Datasets

2015-12-26 | E-GEOD-66796 | ArrayExpress
2014-05-01 | E-GEOD-22831 | ArrayExpress
2014-06-03 | E-GEOD-48892 | ArrayExpress
2014-07-26 | E-GEOD-59552 | ArrayExpress
2014-06-10 | E-GEOD-58091 | ArrayExpress
2014-07-18 | E-GEOD-59277 | ArrayExpress
2016-04-09 | E-GEOD-69894 | ArrayExpress
2014-05-04 | E-GEOD-15027 | ArrayExpress
2012-06-25 | E-GEOD-32484 | ArrayExpress
2014-07-26 | E-GEOD-59553 | ArrayExpress