Transcriptomics

Dataset Information

338

Differential Ago-RIP-Seq for the identification of miR-375 targets in prostate cancer cells


ABSTRACT: The prostate cancer cell line PC-3 was transfected with pre-miR-375 or control. After 48 hours the cells were lysed and immunoprecipitation was performed using anti-panAgo (...) or isotype anti-IgG antibodies overnight. RNA of total lysates and immunoprecipitation fractions was extracted using miRNeasy kit (Qiagen) and libraries were generated using the Stranded Total RNA Sample Prep Kit (Takara Clontech). The experiment was performed in order to identify potential targets of miR-375 in prostate cancer.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens  

SUBMITTER: Julia Romic-Pickl  

PROVIDER: E-MTAB-3691 | ArrayExpress | 2017-01-31

SECONDARY ACCESSION(S): ERP010899

REPOSITORIES: ArrayExpress, ENA

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