Transcriptomics

Dataset Information

302

RNA-seq analysis of parental (wild type) and dcl3 mutant lines of Chlamydomonas reinhardtii


ABSTRACT: DCL3 is the main processor of miRNA precursors in Chlamydomonas reinhardtii. Because of that, and in order identify mRNA targets of miRNA-guided slicer complexes, we used RNAseq of the transcriptome in dcl3 mutant and parental lines.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Chlamydomonas reinhardtii  

SUBMITTER: Bruno ACM Santos   Adrian A Valli   David C Baulcombe  

PROVIDER: E-MTAB-3852 | ArrayExpress | 2016-08-28

SECONDARY ACCESSION(S): ERP011956

REPOSITORIES: ArrayExpress, ENA

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Publications

Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs.

Valli Adrian A AA   Santos Bruno A C M BA   Hnatova Silvia S   Bassett Andrew R AR   Molnar Attila A   Chung Betty Y BY   Baulcombe David C DC  

Genome research 20160311 4


We describe here a forward genetic screen to investigate the biogenesis, mode of action, and biological function of miRNA-mediated RNA silencing in the model algal species,Chlamydomonas reinhardtii Among the mutants from this screen, there were three at Dicer-like 3 that failed to produce both miRNAs and siRNAs and others affecting diverse post-biogenesis stages of miRNA-mediated silencing. The DCL3-dependent siRNAs fell into several classes including transposon- and repeat-derived siRNAs as in  ...[more]

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