Transcriptomics

Dataset Information

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Defining the Nitrogen regulated transcriptome of Mycobacterium smegmatis


ABSTRACT: Nitrogen is essential for microbial growth and its importance is demonstrated by complex regulatory systems used to control the transport, assimilation and metabolism of nitrogen. To gain further insight into the response of mycobacteria to nitrogen limitation, we developed a nitrogen-limited chemostat and compared the transcriptional response of nitrogen-limited cells to nitrogen-replete cells in a continuous culture model at a constant growth rate (0.12 h-1) (td = 5.7 hrs) and 50% oxygen saturation.

INSTRUMENT(S): Illumina MiSeq

SUBMITTER: Michael Petridis  

PROVIDER: E-MTAB-3918 | ArrayExpress | 2015-10-08

SECONDARY ACCESSION(S): ERP012307

REPOSITORIES: ArrayExpress, ENA

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Defining the nitrogen regulated transcriptome of Mycobacterium smegmatis using continuous culture.

Petridis Michael M   Benjak Andrej A   Cook Gregory M GM  

BMC genomics 20151019


Nitrogen is essential for microbial growth and its importance is demonstrated by the complex regulatory systems used to control the transport, assimilation and metabolism of nitrogen. Recent studies are beginning to shed light on how mycobacteria respond to nitrogen limitation and several regulators (e.g., GlnR, PII) have been characterized at a molecular level. However, despite this progress, our knowledge of the transcriptional response of mycobacteria to nitrogen limitation and its regulation  ...[more]

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