Transcriptomics,Genomics

Dataset Information

297

Zea mays Epigenomics


ABSTRACT: RNA-directed DNA methylation (RdDM) in plants is a well-characterized example of RNA interference-related transcriptional gene silencing. To determine the relationships between RdDM and heterochromatin in the repeat-rich maize (Zea mays) genome, we performed whole-genome analyses of several heterochromatic features: dimethylation of lysine 9 and lysine 27 (H3K9me2 and H3K27me2), chromatin accessibility, DNA methylation, and small RNAs; we also analyzed two mutants that affect these processes, mediator of paramutation1 and zea methyltransferase2.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Zea mays  

SUBMITTER: Kelly Dawe  

PROVIDER: E-MTAB-4191 | ArrayExpress | 2014-12-05

SECONDARY ACCESSION(S): SRP047420

REPOSITORIES: ArrayExpress, ENA

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Publications

Accessible DNA and relative depletion of H3K9me2 at maize loci undergoing RNA-directed DNA methylation.

Gent Jonathan I JI   Madzima Thelma F TF   Bader Rechien R   Kent Matthew R MR   Zhang Xiaoyu X   Stam Maike M   McGinnis Karen M KM   Dawe R Kelly RK  

The Plant cell 20141202 12


RNA-directed DNA methylation (RdDM) in plants is a well-characterized example of RNA interference-related transcriptional gene silencing. To determine the relationships between RdDM and heterochromatin in the repeat-rich maize (Zea mays) genome, we performed whole-genome analyses of several heterochromatic features: dimethylation of lysine 9 and lysine 27 (H3K9me2 and H3K27me2), chromatin accessibility, DNA methylation, and small RNAs; we also analyzed two mutants that affect these processes, me  ...[more]

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