Transcriptomics

Dataset Information

46

Transcription profiling of T cells from healthy controls and patients with seasonal allergic rhinitis before and after one year of sublingual immunotherapy


ABSTRACT: We recruit 4 healthy controls (HC) and 4 patients (P) with seasonal allergic rhinitis before (b) sublingual immunotherapy at the same time and also the same patients after one year (a) of sublingual immunotherapy. Peripheral blood mononuclear cells (PBMCs) obtained from patients and controls were challenged with diluent (D) or allergen (A) extracts from birch pollen at a density of 106 cells/mL for 7 days in RPMI 1640 supplemented with 2 mM L-glutamine, 5% human AB serum, 5 μM β– mercaptoethanol and 50 μg/mL gentamicin. CD4+ T cells were isolated using flow cytometry and the quantity and quality of RNA was examined as described before. Gene expression microarrays (Illumina, San Diego, CA, USA) were performed (by using Agilent G4851B SurePrint G3 Hmn 8×60K V2 Microarray Kit).

ORGANISM(S): Homo sapiens  

SUBMITTER: Huan Zhang  

PROVIDER: E-MTAB-4366 | ArrayExpress | 2017-03-31

REPOSITORIES: ArrayExpress

Similar Datasets

2015-01-16 | E-GEOD-43523 | ArrayExpress
2009-10-16 | GSE18574 | GEO
2015-09-01 | E-GEOD-49782 | ArrayExpress
2015-11-12 | E-GEOD-60677 | ArrayExpress
| GSE100386 | GEO
| GSE72324 | GEO
2013-02-05 | E-GEOD-44037 | ArrayExpress
2011-12-30 | E-GEOD-29521 | ArrayExpress
2017-08-23 | E-GEOD-75011 | ArrayExpress
2010-06-05 | E-GEOD-1964 | ArrayExpress