Dataset Information


Transcription profiling by high throughput sequencing of Arabidopsis after treatment with Pseudomonas syringae pathovar tomato

ABSTRACT: This study investigates extent and functional significance of alternative splicing in Arabidopsis thaliana defense against the bacterial pathogen Pseudomonas syringae pv tomato (Pst). We have provided a detailed characterization of the Arabidopsis thaliana transcriptional response to Pseudomonas syringae infection in both susceptible and resistant hosts. We carried out two independent inoculation experiments (biological replicates) for each treatment. Col-0 is susceptible to virulent Pst DC3000 but has a functional RPS4 resistance gene effective against DC3000 expressing AvrRps4

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Steffen Heber  

PROVIDER: E-MTAB-4450 | ArrayExpress | 2013-03-06



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High-throughput RNA sequencing of pseudomonas-infected Arabidopsis reveals hidden transcriptome complexity and novel splice variants.

Howard Brian E BE   Hu Qiwen Q   Babaoglu Ahmet Can AC   Chandra Manan M   Borghi Monica M   Tan Xiaoping X   He Luyan L   Winter-Sederoff Heike H   Gassmann Walter W   Veronese Paola P   Heber Steffen S  

PloS one 20131001 10

We report the results of a genome-wide analysis of transcription in Arabidopsis thaliana after treatment with Pseudomonas syringae pathovar tomato. Our time course RNA-Seq experiment uses over 500 million read pairs to provide a detailed characterization of the response to infection in both susceptible and resistant hosts. The set of observed differentially expressed genes is consistent with previous studies, confirming and extending existing findings about genes likely to play an important role  ...[more]

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