Dataset Information


3'-Tag-Seq of Drosophila melanogaster embryos from 82 wild-type strains from the Drosophia Genetic Reference Panel (DGRP) over three time-points during embryogenesis

ABSTRACT: Sequencing of the most 3' end of mRNAs (3'-Tag-Seq) of 82 wild type strains from the Drosophila Genetic reference Panel (DGRP) during embryogenesis was performed. Three different time points (2-4hrs, 6-8hrs and 10 to 12rs after laying) were assayed. 14 biological replicates were performed from independent embryo collections.

INSTRUMENT(S): Illumina HiSeq2000

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Ewan Birney   Charles Girardot   Nils Koelling   Enrico Cannavo   Eileen Furlong 

PROVIDER: E-MTAB-4722 | ArrayExpress | 2017-01-10



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Embryonic development is driven by tightly regulated patterns of gene expression, despite extensive genetic variation among individuals. Studies of expression quantitative trait loci (eQTL) indicate that genetic variation frequently alters gene expression in cell-culture models and differentiated tissues. However, the extent and types of genetic variation impacting embryonic gene expression, and their interactions with developmental programs, remain largely unknown. Here we assessed the effect o  ...[more]

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