Transcriptomics

Dataset Information

362

Single cell RNA-sequencing of Spike-ins and mESC using SMARTer on C1 System


ABSTRACT: In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific methods. In this dataset, we perform SMARTer method on Fluidigm C1 system and generate single-cell libraries using Nextera XT kit

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus  

SUBMITTER: Guy Emerton   Valentine Svensson  

PROVIDER: E-MTAB-5483 | ArrayExpress | 2017-03-28

SECONDARY ACCESSION(S): ERP022289

REPOSITORIES: ArrayExpress, ENA

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