Genomics

Dataset Information

336

Simple sequence repeat identification for RBOHB gene in multiple varieties of Oryza sativa


ABSTRACT: Studies have shown that Respiratory Burst Oxidase Homolog B (RBOHB) are involved in stress response in rice plants. Primers were developed for amplification via Polymerase Chain Reaction (PCR) of a region that contained a simple sequence repeat (SSR) in RBOHB. PCR was performed on 6 different varieties of Oryza sativa. PCR product was sequenced on an ABI 3730 capillary sequence machine. Sequence data was aligned to observe differences in SSR length between each rice variety.

INSTRUMENT(S): Promega PCR Master Mix. Bio-Rad Thermal Cycler, AB 3730 Genetic Analyzer, Qiagen DNeasy, 3M Sodium Acetate, 100% Ethanol

SUBMITTER: Matthew Geniza   Pankaj Jaiswal  

PROVIDER: E-MTAB-5560 | ArrayExpress | 2017-12-31

SECONDARY ACCESSION(S): ERP022285

REPOSITORIES: ArrayExpress, ENA

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