Transcriptomics

Dataset Information

49

Affymetrix whole transcriptome gene expression analysis of ER-stressed Jurkat cells


ABSTRACT: We induced ER stress in Jurkat T-cells with dithiothreitol (DTT), which reduces the protein disulfide bonds and causes the accumulation of misfolded proteins in the ER lumen. For assessment of DTT effects on cells, we performed Affymetrix whole transcriptome gene expression analysis. The cells were treated for 6 hours with 2.5 mM Dithiothreitol (Sigma-Aldrich). Total RNA was isolated from DTT-treated (2 replicates) and control (2 replicates) Jurkat cells and hybridized to a gene expression arrays (GeneChip Gene 1.0 ST Array System; Affymetrix, Santa Clara, CA).

ORGANISM(S): Homo Sapiens

SUBMITTER: Alexey Moskovtsev  

PROVIDER: E-MTAB-5574 | ArrayExpress | 2017-04-15

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MTAB-5574.idf.txt Idf
E-MTAB-5574.processed.1.zip Processed
E-MTAB-5574.raw.1.zip Raw
E-MTAB-5574.sdrf.txt Txt
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