Transcriptomics

Dataset Information

333

RNA-Seq analysis of MCV-miR-M1 mimic-transfected HEK293 cells


ABSTRACT: To determine transcriptomic changes in cellular targets induced by MCV-miR-M1. Briefly, HEK293 cells were transfected with either MCV-miR-M1-5p, MCV-miR-M1-3p or control mimic (Thermo Fisher Scientific) prior to RNA extraction and confirmation of MCV miRNA 5p and 3p expression via stem loop qRT-PCR. Total RNA libraries were prepared using TruSeq Stranded Total RNA Sample Prep Kit (Illumina, USA) and the TruSeq cDNA libraries were analysed via Illumina HiSeq2500 paired end 100bp.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens  

SUBMITTER: James R Boyne  

PROVIDER: E-MTAB-6526 | ArrayExpress | 2018-03-31

SECONDARY ACCESSION(S): ERP107000

REPOSITORIES: ArrayExpress, ENA

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