Transcriptomics

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SynSTARR-seq with Glucocorticoid Receptor synthetic binding sites in GR18 cell line


ABSTRACT: We performed STARR-seq with synthetic libraries (synSTARR-seq) in GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment. The synthetic libraries are variants of the glucocorticoid receptor binding sites (GBS). The "GBS half site" library contains 8 consecutive randomized nucleotides within the core binding sites, while the "Cgt/Sgk library" contains 5 consecutive randomized nucleotides on the flank of the GBS.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens  

SUBMITTER: Sebastiaan Meijsing  Morgane Thomas-Chollier    

PROVIDER: E-MTAB-6737 | ArrayExpress | 2018-10-29

SECONDARY ACCESSION(S): ERP108474

REPOSITORIES: ArrayExpress, ENA

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