Transcriptomics

Dataset Information

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Single-cell RNA-seq of naive and primed human embryonic stem cells


ABSTRACT: This study aims to profile the transcriptomes of single naive and primed human embryonic stem cells. Cells from the H9 line were cultured to select for naive or primed phenotypes, and a sequencing library was generated from each single cell using the Smart-seq2 method. This was repeated for multiple experimental batches, i.e., independent cultures. Batch 1 consists of sequencing runs 2383 and 2384; batch 2 consists of runs 2678, 2679, 2739 and 2740; and batch 3 consists of runs 2780 and 2781. Transcriptional profiles for all cells were obtained from the sequencing data and used to explore substructure and heterogeneity in the population for each phenotype.

INSTRUMENT(S): Illumina HiSeq 2500, BD FACSAria cell sorter

ORGANISM(S): Homo sapiens  

SUBMITTER: Aaron TL Lun  

PROVIDER: E-MTAB-6819 | ArrayExpress | 2018-07-26

SECONDARY ACCESSION(S): ERP108653

REPOSITORIES: ArrayExpress, ENA

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