Genomics

Dataset Information

29

Targeted DNA-seq of Human HepG2 cells following editing with CRISPR/Cas9 upon chromatin modulation with TSA and EZH2i


ABSTRACT: We sought to characterise how chromatin states affected CRISPR/Cas9 activity and the induced indel profiles. We used two different doses of Trichostatin and two different doses of a EZH2 inhibitor to modulate the chromatin state and then performed targeted DNA-seq of selected sgRNA target regions to identify the indels.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Homo sapiens  

SUBMITTER: Paola Scaffidi   Anob Chakrabarti  

PROVIDER: E-MTAB-7091 | ArrayExpress | 2018-12-13

SECONDARY ACCESSION(S): ERP110147

REPOSITORIES: ArrayExpress, ENA

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