Genomics

Dataset Information

0

DNA-seq of 5fC enriched fragments based


ABSTRACT: These dataset serve to illustrate the 5fC pull down protocol developed in our lab.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus  

SUBMITTER: Guillem Portella  

PROVIDER: E-MTAB-7307 | ArrayExpress | 2019-12-01

SECONDARY ACCESSION(S): ERP111530

REPOSITORIES: ArrayExpress, ENA

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Publications

Chemical biology of genomic DNA: minimizing PCR bias.

McInroy Gordon R GR   Raiber Eun-Ang EA   Balasubramanian Shankar S  

Chemical communications (Cambridge, England) 20141001 81


The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe. ...[more]

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