Transcriptomics

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Nanopore RNA-seq of polyA+ RNA from HAP1 and HEK293 cell lines


ABSTRACT: Sequencing was performed to assess the ability of Nanopore direct cDNA and native RNA sequencing to characterise human transcriptomes. Total RNA was extracted from either HAP1 or HEK293 cells, and the polyA+ fraction isolated using oligodT dynabeads. Libraries were prepared using Oxford Nanopore Technologies (ONT) kits according to manufacturers instructions. Samples were then sequenced on ONT R9.4 flow cells to generate fast5 raw reads in the ONT MinKNOW software. Fast5 reads were then base-called using the ONT Albacore software to generate Fastq reads.

INSTRUMENT(S): MinION

ORGANISM(S): Homo sapiens  

SUBMITTER: Shobbir Hussain  Charlotte Soneson     

PROVIDER: E-MTAB-7778 | ArrayExpress | 2019-03-13

SECONDARY ACCESSION(S): ERP114244

REPOSITORIES: ArrayExpress, ENA

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