Transcriptomics

Dataset Information

4

Transcription profiling analysis of human global and individual mRNA translational efficiencies


ABSTRACT: A cell synchronization protocol was established in which global and individual mRNA translational efficiencies could be examined. While global translational efficiency was reduced in mitotic cells, approximately 3% of mRNAs remained predominantly associated with large polysomes during mitosis, as determined by cDNA microarray analyses. The 5 noncoding regions of six mRNAs were shown to contain internal ribosome entry sites (IRES). However, not all known mRNAs that contain IRES elements were actively translated during mitosis, arguing that specific IRES sequences are differentially regulated during mitosis.

ORGANISM(S): Homo sapiens  

SUBMITTER: Janos Demeter   Peter Sarnow  

PROVIDER: E-SMDB-2546 | ArrayExpress | 2005-10-31

REPOSITORIES: ArrayExpress

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Publications

Preferential translation of internal ribosome entry site-containing mRNAs during the mitotic cycle in mammalian cells.

Qin Xiaoli X   Sarnow Peter P  

The Journal of biological chemistry 20040121 14


A cell synchronization protocol was established in which global and individual mRNA translational efficiencies could be examined. While global translational efficiency was reduced in mitotic cells, approximately 3% of mRNAs remained predominantly associated with large polysomes during mitosis, as determined by cDNA microarray analyses. The 5'-non-coding regions of six mRNAs were shown to contain internal ribosome entry sites (IRES). However, not all known mRNAs that contain IRES elements were ac  ...[more]

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