Transcriptomics

Dataset Information

49

Transcription profiling of human wild type CMV vs mock infected HFFs


ABSTRACT: Polyadenylated RNA from 10^7 confluent HFs was purified by using Oligotex (QIAGEN, Valencia, Calif.) from Trizol (Invitrogen)-extracted total RNA. This RNA was prepared, reverse transcribed, labeled with Cy3-dUTP or Cy5-dUTP (Amersham, Little Chalfont, Buckinghamshire, United Kingdom) by random primed synthesis with DNA pol I Klenow (Amersham Life Science, Inc., Cleveland, Ohio), and hybridized to spotted, human cDNA microarrays as previously described. Sequence-verified human cDNA microarrays (HE and HG series, 31,000 spots; HD51 series, 17,000 spots) were produced at Stanford. Images were collected by using a GenePix 4000B microarray scanner, manually flagged to eliminate poor spots and analyzed by GenePix Pro 2.0 (Axon, Union City, Calif.)

ORGANISM(S): Homo sapiens  

SUBMITTER: Janos Demeter  Davide Abate     

PROVIDER: E-SMDB-2957 | ArrayExpress| 2014-06-04

REPOSITORIES: ArrayExpress

altmetric image

Publications

Major human cytomegalovirus structural protein pp65 (ppUL83) prevents interferon response factor 3 activation in the interferon response.

Abate Davide A DA   Watanabe Shinya S   Mocarski Edward S ES  

Journal of virology 20041001 20


We have identified a cytomegalovirus virion protein capable of modulating the rapid induction of an interferon-like response in cells that follows virus binding and penetration. Functional genomics revealed a role for the major cytomegalovirus structural protein, pp65 (ppUL83), in counteracting this response. The underlying mechanism involves a differential impact of this structural protein on the regulation of interferon response factor 3 (IRF-3). In contrast, NF-kappaB is activated independent  ...[more]

Similar Datasets

2014-06-04 | E-SMDB-2962 | ArrayExpress
2014-06-04 | E-SMDB-2959 | ArrayExpress
2014-06-04 | E-SMDB-2960 | ArrayExpress
2014-06-04 | E-SMDB-2961 | ArrayExpress
2011-10-18 | E-GEOD-4015 | ArrayExpress
2005-12-21 | GSE3887 | GEO
2011-10-18 | E-GEOD-3887 | ArrayExpress
2014-05-02 | E-GEOD-8502 | ArrayExpress
2008-12-21 | GSE8502 | GEO
2006-10-31 | GSE3540 | GEO