Transcriptomics

Dataset Information

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Gene expression profile in breast cancer cells


ABSTRACT: Human estrogen-responsive breast cancer cell line MCF-7 wt were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (Ct-ER-beta and Nt-ER-beta) as previously described. MCF7 wt and beta clone cells were cultured in steroid-free medium for 5 days and then were treated with 10nM of 17-beta-estradiol, or vehicle (ETOH). RNA was extracted after 2h, 4h and 8h of stimulation with 17-ß-estradiol 10 nM (+E2) or ethanol vehicle . Total RNA extracted by Ct-ER-beta and Nt-ER-beta cells were pooled (TAP-ER-beta). For mRNA expression profiling, 500 ng total RNA were reverse transcribed and used for synthesis of cDNA and biotinylated cRNA. Finally cRNA were hybridized for 18 hours on Illumina HumanHT-12 v3.0 BeadChips and after scanning, data analysis was performed.

ORGANISM(S): Homo sapiens  

SUBMITTER: Alessandro Weisz   

PROVIDER: E-TABM-1051 | ArrayExpress| 2014-05-02

REPOSITORIES: ArrayExpress

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Publications

Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation.

Grober Oli M V OM   Mutarelli Margherita M   Giurato Giorgio G   Ravo Maria M   Cicatiello Luigi L   De Filippo Maria Rosaria MR   Ferraro Lorenzo L   Nassa Giovanni G   Papa Maria Francesca MF   Paris Ornella O   Tarallo Roberta R   Luo Shujun S   Schroth Gary P GP   Benes Vladimir V   Weisz Alessandro A  

BMC genomics 20110114


BACKGROUND: Estrogen receptors alpha (ERα) and beta (ERβ) are transcription factors (TFs) that mediate estrogen signaling and define the hormone-responsive phenotype of breast cancer (BC). The two receptors can be found co-expressed and play specific, often opposite, roles, with ERβ being able to modulate the effects of ERα on gene transcription and cell proliferation. ERβ is frequently lost in BC, where its presence generally correlates with a better prognosis of the disease. The identification  ...[more]

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