Transcription profiling of fission yeast in response to changes in copper and iron levels
ABSTRACT: Recent studies in comparative genomics demonstrate that inter-species comparisons represent a powerful tool for revealing both conserved and specialized biological processes across large evolutionary distances. All cells need to adjust to environmental fluctuations in metal levels since both metal excess and deficit are detrimental. Here, we explore the conservation of metal homoeostasis in two distantly related yeasts, examining genome-wide gene expression responses to changing copper and iron levels in budding and fission yeast using DNA microarrays
<h4>Background</h4>Recent studies in comparative genomics demonstrate that interspecies comparison represents a powerful tool for identifying both conserved and specialized biologic processes across large evolutionary distances. All cells must adjust to environmental fluctuations in metal levels, because levels that are too low or too high can be detrimental. Here we explore the conservation of metal homoeostasis in two distantly related yeasts.<h4>Results</h4>We examined genome-wide gene expres ...[more]
Project description:Transcription profiling of fission yeast /yox1/ deletion and genome wide location analysis of Yox1p and Cdc10p transcription factors reveal a negative feedback interaction: /yox1 /is transcriptionally activated by MBF, and Yox1p in turn transcriptionally represses the MBF target genes (including /yox1 /itself).